TET Hydroxylase Activity Quantification Kit (Colorimetric) (ab156912)


  • Product name
    TET Hydroxylase Activity Quantification Kit (Colorimetric)
    See all TET1 kits
  • Sample type
    Cell culture supernatant, Tissue, Nuclear Extracts, Purified protein
  • Assay type
    Enzyme activity
  • Sensitivity
    > 20 ng
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Plants, Mammal, Fungi
  • Product overview

    TET Hydroxylase Activity Quantification Kit (Colorimetric) (ab156912) is suitable for measuring the activity/inhibition of total 5mC hydroxylase TET enzyme using nuclear extracts or purified TET isoforms (TET 1-3) from a broad range of species such as mammalian, plant, fungal, and bacterial, in a variety of forms including, but not limited to cultured cells, fresh and frozen tissues.

  • Notes

    DNA methylation occurs by the covalent addition of a methyl group at the 5-carbon of the cytosine ring by DNA methyltransferases, resulting in 5-methylcytosine (5mC). In somatic cells, 5mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG, whereas in embryonic stem (ES) cells, a substantial amount of 5mC is also observed in non-CpG contexts. The biological importance of 5mC as amajor epigenetic modification in phenotype and gene expression has been recognized widely.

    5-hydroxymethylcytosine (5hmC), as a sixth DNA base with functions in transcription regulation, has been detected to be abundant in human and mouse brain and embryonic stem (ES) cells. In mammals, it can be generated by oxidation of 5mC, a reaction mediated by the ten-eleven translocation (TET) family of 5mC-hydroxylases.

    The TET family of 5mC hydroxylases includes TET1, TET2 and TET3. These TET proteins may promote DNA demethylation by binding to CpG-rich regions to prevent unwanted DNA methyltransferase activity, and by converting 5mC to 5hmC and further to 5-carboxylcytosine (5-caC) through hydroxylase activity. It was shown that genomic 5hmC level correlates to TET hydroxylase activity. In addition, TET1 was shown to have dual functions in transcription activation and repression by binding different target genes in ES cells. TET1 is also a fusion partner of the MLL gene in acute myeloid leukemia and is considered an oncoprotein. TET2 is found to be frequently mutated in leukemia and considered to act as tumor suppressor. TET3 has been demonstrated to play a unique role for DNA methylation reprogramming processes in the mammalian zygote. Thus, activating tumor suppressor TET enzymes such as TET2 or inhibiting oncoprotein TET enzymes such as TET1 would be important in benefiting cancer diagnostics and developing new target-based cancer therapeutics.

  • Tested applications
    Suitable for: Functional Studiesmore details


  • Storage instructions
    Please refer to protocols.
  • Components 48 tests 96 tests
    10X TET Substrate 1 x 10µl 1 x 20µl
    10X Wash Buffer 1 x 14ml 1 x 28ml
    8-Well Assay Strips (with Frame) 1 x 6 units 1 x 12 units
    Binding Solution 1 x 5ml 1 x 10ml
    Capture Antibody, 1000 µg/mL 1 x 4µl 1 x 8µl
    Co-factor 1 1 x 25µl 1 x 50µl
    Co-factor 2 1 x 25µl 1 x 50µl
    Co-factor 3 1 x 25µl 1 x 50µl
    Detection Antibody, 400 µg/mL 1 x 8µl 1 x 16µl
    Developer Solution 1 x 5ml 1 x 10ml
    Enhancer Solution 1 x 8µl 1 x 16µl
    Stop Solution 1 x 3ml 1 x 6ml
    TET Assay Buffer 1 x 3ml 1 x 6ml
    TET Assay Standard, 20 µg/mL 1 x 10µl 1 x 20µl
  • Research areas
  • Function
    Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). Might initiate a process leading to cytosine demethylation through deamination into 5-hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification.
  • Tissue specificity
    Expressed in fetal heart, lung and brain, and in adult skeletal muscle, thymus and ovary. Not detected in adult heart, lung or brain.
  • Involvement in disease
    Note=A chromosomal aberration involving TET1 may be a cause of acute leukemias. Translocation t(10;11)(q22;q23) with MLL. This is a rare chromosomal translocation 5' MLL-TET1 3'.
  • Sequence similarities
    Belongs to the TET family.
    Contains 1 CXXC-type zinc finger.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • bA119F7.1
    • CXXC finger 6
    • CXXC zinc finger 6
    • CXXC-type zinc finger protein 6
    • CXXC6
    • LCX
    • Leukemia-associated protein with a CXXC domain
    • Methylcytosine dioxygenase TET1
    • Ten eleven translocation 1
    • Ten-eleven translocation 1 gene protein
    • Tet methylcytosine dioxygenase 1
    • Tet oncogene 1
    • TET1
    • TET1_HUMAN
    see all


Our Abpromise guarantee covers the use of ab156912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.


  • Demonstration of high sensitivity and specificity of the TET activity assay achieved by using nuclear extracts with TET Hydroxylase Activity Quantification Kit (Colorimetric) (ab156912). Nuclear extracts were prepared from Mouse ES cells.

  • Illustrated standard curve generated with the TET assay standard.
  • Demonstration of high sensitivity and specificity of the TET1 activity/inhibition assay achieved by using recombinant TET1 with the TET Hydroxylase Activity Quantification Kit (Colorimetric) (ab156912).



ab156912 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The product formed after TET enzyme-reaction is hydroxymethylated DNA, which will be bound and detected by anti-5-hmC Antibody included in the kit.


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