Overview

  • Product nameAnti-TET1 antibody
    See all TET1 primary antibodies
  • Description
    Rabbit polyclonal to TET1
  • SpecificityPlease note this antibody does not give a signal with endogenous lysates.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human TET1 aa 1350-1450 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q8NFU7

  • Positive control
    • This antibody gave a positive signal in HEK293 Over-expressing Full length TET1

Properties

Applications

Our Abpromise guarantee covers the use of ab105475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 230 kDa (predicted molecular weight: 235 kDa).

Target

  • FunctionDioxygenase that catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays a role in embryonic stem (ES) cell maintenance and inner cell mass (ICM) cell specification, possibly by participating in DNA demethylation. Specifically binds 5mC, a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. 5mC is present in ES cells and is enriched in the brain, especially in Purkinje neurons. The clear function of hmC is still unclear but it could constitute an intermediate component in cytosine demethylation. A role of hmC in DNA demethylation is supported by TET1 function in ES cell maintenance, which is required to prevent NANOG hypermethylation and maintain NANOG expression in ES cells.
  • Tissue specificityExpressed in fetal heart, lung and brain, and in adult skeletal muscle, thymus and ovary. Not detected in adult heart, lung or brain.
  • Involvement in diseaseNote=A chromosomal aberration involving TET1 may be a cause of acute leukemias. Translocation t(10;11)(q22;q23) with MLL. This is a rare chromosomal translocation 5' MLL-TET1 3'.
  • Sequence similaritiesBelongs to the TET family.
    Contains 1 CXXC-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA119F7.1 antibody
    • CXXC 6 antibody
    • CXXC finger 6 antibody
    • CXXC type zinc finger protein 6 antibody
    • CXXC zinc finger 6 antibody
    • CXXC-type zinc finger protein 6 antibody
    • CXXC6 antibody
    • KIAA1676 antibody
    • LCX antibody
    • Leukemia associated protein with a CXXC domain antibody
    • Leukemia-associated protein with a CXXC domain antibody
    • Methylcytosine dioxygenase TET1 antibody
    • Ten eleven translocation 1 antibody
    • Ten eleven translocation 1 gene protein antibody
    • Ten eleven translocation 1 gene protein homolog antibody
    • Ten-eleven translocation 1 gene protein antibody
    • Tet 1 antibody
    • Tet methylcytosine dioxygenase 1 antibody
    • Tet oncogene 1 antibody
    • TET1 antibody
    • TET1_HUMAN antibody
    see all

Anti-TET1 antibody images

  • All lanes : Anti-TET1 antibody (ab105475) at 1 µg/ml

    Lane 1 : HEK293 Over-expressing Full length TET1
    Lane 2 : HEK293 Over-expressing TET1CD

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 235 kDa
    Additional bands at : 230 kDa. We are unsure as to the identity of these extra bands.

    This image is courtesy of Hongjun Song, Johns Hopkins Univ School of Med, United States

References for Anti-TET1 antibody (ab105475)

ab105475 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (embryonic stem cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification embryonic stem cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Mar 03 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"