Anti-TET3 antibody - C-terminal (ab153724)

Overview

  • Product name
    Anti-TET3 antibody - C-terminal
    See all TET3 primary antibodies
  • Description
    Rabbit polyclonal to TET3 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1456-1660 of Human TET3 (Uniprot ID: O43151).

  • Positive control
    • Human TET3-transfected 293T cell lysate with DDDDK tag; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 179 kDa.
ICC/IF 1/100 - 1/1000.
IP 1/500 - 1/1000.

Target

  • Function
    Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC).
  • Tissue specificity
    Expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes.
  • Sequence similarities
    Belongs to the TET family.
  • Developmental stage
    Expressed in fetal brain but not adult brain.
  • Information by UniProt
  • Database links
  • Alternative names
    • hCG_40738 antibody
    • KIAA0401 antibody
    • Methylcytosine dioxygenase TET3 antibody
    • Probable methylcytosine dioxygenase TET3 antibody
    • Tet methylcytosine dioxygenase 3 antibody
    • Tet oncogene family member 3 antibody
    • Tet3 antibody
    • TET3_HUMAN antibody
    see all

Images

  • All lanes : Anti-TET3 antibody - C-terminal (ab153724) at 1/1000 dilution

    Lane 1 : Cell lysate/extract of transfected 293T cells.
    Lane 2 : Control of preimmune Rabbit IgG at 2 µg
    Lane 3 : Immunoprecipitation of TET3 by ab153724
    at 2 µg
  • All lanes : Anti-TET3 antibody - C-terminal (ab153724) at 1/5000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : Human TET3-transfected 293T whole cell lysate with DDDDK tag



    Lysates/proteins at 30 µg per lane.

    Predicted band size: 179 kDa



    5 % SDS-PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling TET3 with ab153724 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

ab153724 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E10.5 embryo)
Permeabilization
Yes - 0.1% triton
Specification
E10.5 embryo
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Mar 04 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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