Anti-Tetanus Toxin antibody [TetE3] (ab64755)

Overview

  • Product name
    Anti-Tetanus Toxin antibody [TetE3]
    See all Tetanus Toxin primary antibodies
  • Description
    Mouse monoclonal [TetE3] to Tetanus Toxin
  • Tested applications
    Suitable for: Neutralising, WB, ELISAmore details
  • Species reactivity
    Reacts with Clostridium tetani.
  • Immunogen

    Supernatant from C. tetani.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab64755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    Neutralising activity, in vivo assay: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight (precursor): 151 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln-|-Phe-77 bond of synaptobrevin-2. The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation.
    • Cellular localization
      Secreted
    • Alternative names
      • Tentoxylysin antibody
      • Tetanus toxin chain H antibody
      • Tetanus toxin chain L antibody
      • Tetanus toxin heavy chain antibody
      • Tetanus toxin light chain antibody
      see all

    References

    This product has been referenced in:
    • Kalb SR  et al. Extraction of BoNT/A, /B, /E, and /F with a single, high affinity monoclonal antibody for detection of botulinum neurotoxin by Endopep-MS. PLoS One 5: (2010). Read more (PubMed: 20808925) »

    See 1 Publication for this product

    Customer reviews and Q&As

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    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Retina slice)
    Specification
    Retina slice
    Username

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    Verified customer

    Submitted Jun 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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