Anti-Tetanus Toxin antibody [TetE3] (ab64755)

Overview

  • Product nameAnti-Tetanus Toxin antibody [TetE3]
    See all Tetanus Toxin primary antibodies
  • Description
    Mouse monoclonal [TetE3] to Tetanus Toxin
  • Tested applicationsSuitable for: Neutralising, WB, ELISAmore details
  • Species reactivity
    Reacts with Clostridium tetani.
  • Immunogen

    Supernatant from C. tetani.

Properties

Applications

Our Abpromise guarantee covers the use of ab64755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    Neutralising activity, in vivo assay: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight (precursor): 151 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceTetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln-|-Phe-77 bond of synaptobrevin-2. The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation.
    • Cellular localizationSecreted
    • Alternative names
      • Tentoxylysin antibody
      • Tetanus toxin chain H antibody
      • Tetanus toxin chain L antibody
      • Tetanus toxin heavy chain antibody
      • Tetanus toxin light chain antibody
      see all

    References for Anti-Tetanus Toxin antibody [TetE3] (ab64755)

    This product has been referenced in:
    • Kalb SR  et al. Extraction of BoNT/A, /B, /E, and /F with a single, high affinity monoclonal antibody for detection of botulinum neurotoxin by Endopep-MS. PLoS One 5: (2010). Read more (PubMed: 20808925) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.
    Yes, as soon as your Abreview is submitted the discount code can be used. Thus, you can go ahead now and use it on a future order before the expiration date.
    Thank you so much for submitting your Abreview!
    I ...

    Read More

    Thank you for submitting an Abreview of ab64755. As you may have noticed, your review has now been published on our website.
    Since you obtained poor results using the antibody in an untested application, we would like to follow up on this to see i...

    Read More
    Application IHC - Wholemount
    Sample Mouse Tissue (Retina slice)
    Specification Retina slice
    Username

    Abcam user community

    Verified customer

    Submitted Jun 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"