The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
Neutralising activity, in vivo assay: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight (precursor): 151 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceTetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln-|-Phe-77 bond of synaptobrevin-2.
The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation.
Tetanus toxin chain H antibody
Tetanus toxin chain L antibody
Tetanus toxin heavy chain antibody
Tetanus toxin light chain antibody
References for Anti-Tetanus Toxin antibody [TetE3] (ab64755)
This product has been referenced in:
Kalb SR et al. Extraction of BoNT/A, /B, /E, and /F with a single, high affinity monoclonal antibody for detection of botulinum neurotoxin by Endopep-MS. PLoS One5: (2010).
Read more (PubMed: 20808925) »