Overview

  • Product name
    Anti-Tetranectin antibody [5B7]
    See all Tetranectin primary antibodies
  • Description
    Mouse monoclonal [5B7] to Tetranectin
  • Specificity
    ab51883 is specific for amino acids 17-181 of human tetranectin monomer.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tetranectin purified from human citrate plasma and coupled to PPD. Boosted before fusion with recombinant tetranectin produced in E. coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab51883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
ELISA 1/25.
IHC-Fr 1/40.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.
  • Tissue specificity
    Found in plasma.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin domain family 3 member B antibody
    • C-type lectin domain family 3 member B antibody
    • Clec3b antibody
    • Plasminogen kringle 4 binding protein antibody
    • Plasminogen kringle 4-binding protein antibody
    • TETN_HUMAN antibody
    • Tetranectin (plasminogen binding protein) antibody
    • Tetranectin antibody
    • TN antibody
    • TNA antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of human neutrophils labelling Tetranectin (red) with ab51883. Counterstained with DAPI. A - DAPI, B - Tetranectin, C - Overlay.

  • All lanes : Anti-Tetranectin antibody [5B7] (ab51883) at 5 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 25 kDa (possible post-translational modification),55 kDa. We are unsure as to the identity of these extra bands.
  • Lane 1 :
    Lanes 2 - 3 : Anti-Tetranectin antibody [5B7] (ab51883)

    Lane 1 : Marker
    Lane 2 : Human serum at 20 µl
    Lane 3 : Bovine serum at 20 µl


    Predicted band size : 22 kDa

References

This product has been referenced in:
  • Richens JL  et al. Practical detection of a definitive biomarker panel for Alzheimer's disease; comparisons between matched plasma and cerebrospinal fluid. Int J Mol Epidemiol Genet 5:53-70 (2014). ELISA ; Human . Read more (PubMed: 24959311) »
  • Pernemalm M  et al. Use of narrow-range peptide IEF to improve detection of lung adenocarcinoma markers in plasma and pleural effusion. Proteomics 9:3414-24 (2009). WB ; Human . Read more (PubMed: 19609957) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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