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Rabbit polyclonal to Tetraspanin-33
Predicted to work with:
Mouse, Cow, Zebrafish
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Tetraspanin-33.
(Peptide available as
This antibody gave a positive signal in Human Spleen Tissue Lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 mg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Use a concentration of 5 µg/ml.
Plays an important role in normal erythropoiesis. It has a role in the differentiation of erythroid progenitors.
Predominantly expressed in erythroblasts.
Belongs to the tetraspanin (TM4SF) family.
Information by UniProt
Western blot - Tetraspanin-33 antibody (ab79130)
Anti-Tetraspanin-33 antibody (ab79130) at 1 µg/ml + Human spleen tissue lysate - total protein (
) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
30 kDa (
why is the actual band size different from the predicted?
Exposure time :
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tetraspanin-33 antibody (ab79130)
IHC image of ab79130 staining in Hodgekin's Lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond
TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79130, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"