Anti-Tetraspanin 9 antibody (ab43620)

Overview

  • Product nameAnti-Tetraspanin 9 antibody
    See all Tetraspanin 9 primary antibodies
  • Description
    Mouse polyclonal to Tetraspanin 9
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment:

    RSFSRLRRNPQSADSGADTSGRRDSADKC

    , corresponding to amino acids 23-51 of Human Tetraspanin 9

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 27 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

IHC-P Use at an assay dependent concentration. PubMed: 14145

Target

  • Tissue specificityExpressed in megakaryocytes and platelets (at protein level).
  • Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationMembrane. Colocalizes with GP6 in tetraspanin microdomains on the platelet surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6720474K14Rik antibody
    • 9430079M16Rik antibody
    • AU018597 antibody
    • NET 5 antibody
    • NET5 antibody
    • New EST tetraspan 5 antibody
    • PP1057 antibody
    • Tetraspan NET 5 antibody
    • Tetraspan NET-5 antibody
    • Tetraspanin-9 antibody
    • Transmembrane 4 superfamily member tetraspan NET 5 antibody
    • TSN9_HUMAN antibody
    • Tspan-9 antibody
    • TSPAN9 antibody
    see all

References for Anti-Tetraspanin 9 antibody (ab43620)

ab43620 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Lung)
Loading amount 20 µg
Specification Lung
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
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Verified customer

Submitted Jun 04 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Normal liver)
Specification Normal liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: edta
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Apr 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"