Overview

  • Product nameAnti-TEX12 antibody
    See all TEX12 primary antibodies
  • Description
    Rabbit polyclonal to TEX12
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: MANHLVKPDN RNCKRPRELE SPVPDSPQLS SLGKSDSSFS EISGLFYKDE ALEKDLNDVS, corresponding to amino acids 2-61 of Human TEX12.

  • Positive control
    • Human testis tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab122455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-TEX12 antibody images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in centrosome. Recommended concentration of ab122455 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1 :
    Lanes 2 - 3 : Anti-TEX12 antibody (ab122455)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing TEX12 with a C-terminal myc-DDK tag (~3.1kDa)
  • ab122455, at 1/200 dilution, staining TEX12 in paraffin embedded Human testis tissue by Immunohistochemistry.

References for Anti-TEX12 antibody (ab122455)

ab122455 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"