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Rabbit polyclonal to TEX12
antigen sequence: MANHLVKPDN RNCKRPRELE SPVPDSPQLS SLGKSDSSFS EISGLFYKDE ALEKDLNDVS, corresponding to amino acids 2-61 of Human TEX12.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/100 - 1/250.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Information by UniProt
- Testis expressed gene 12 antibody
- Testis expressed sequence 12 protein variant 3 antibody
- Testis-expressed sequence 12 protein antibody
Immunocytochemistry/ Immunofluorescence - Anti-TEX12 antibody (ab122455)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in centrosome. Recommended concentration of ab122455 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-TEX12 antibody (ab122455)
Lanes 2-3 : Anti-TEX12 antibody (ab122455)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing TEX12 with a C-terminal myc-DDK tag (~3.1kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEX12 antibody (ab122455)
ab122455, at 1/200 dilution, staining TEX12 in paraffin embedded Human testis tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"