Anti-Tex14 antibody (ab42414)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab42414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • RelevanceTestis-expressed gene 14 (TEX14) is a novel protein that localizes to germ cell intercellular bridges. In the absence of TEX14, intercellular bridges are not observed. Spermatogenesis in Tex14–/– mice progresses through the transit amplification of diploid spermatogonia and the expression of early meiotic markers but halts before the completion of the first meiotic division.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Cancer/testis antigen 113 antibody
    • CT113 antibody
    • Inactive serine/threonine protein kinase TEX14 antibody
    • Protein kinase like protein SgK307 antibody
    • Sugen kinase 307 antibody
    • Testis expressed Protein 14 antibody
    • Testis expressed sequence 14 antibody
    • Testis expressed sequence 14 protein antibody
    see all

Anti-Tex14 antibody images

  • IHC image of Tex14 staining in mouse normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42414, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-Tex14 antibody (ab42414)

ab42414 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis, postnatal day 6)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis, postnatal day 6
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

Submitted Jul 14 2016

Thank you for contacting Abcam. Neither ab42414 or ab41733 have been tested on human samples and based on sequence homology between the mouse immunogen sequence and the human sequence, I do not think that they would react. However, ab74835 will react w...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Testis (20 days post-partum))
Specification Testis (20 days post-partum)
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"