The Abcam Texas Red® Fast Conjugation Kit (ab195225) provides an easy-to-use, one step procedure that allows researchers to covalently label proteins, peptides and other biomolecules with Texas Red® with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.
Texas Red® is a red fluorescent dye, used in histology, in fluorescence microscopy applications and in immunohistochemistry. It has a strong absorption at 595nm, with a high fluorescence at 615nm (extinction coefficient 8.0 x104 cm-1M-1) and high quantum yield. Labeled antibodies can be used in many techniques such as flow cytometry.
Texas Red® is a registered trademark of Life Technologies
The kit comes in 3 sizes for labelling recommended amounts of antibody:
300 μg containing 3 reactions (each for 100 - 200 μg)
1 mg containing 1 reaction (for 1 - 2 mg)
30 μg containing 3 reactions (each for 10 - 20 μg)
The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.
|Components||30 µg||300 µg||1 mg|
|Texas Red Conjugation Mix||3 vials||3 vials||1 vial|
|Texas Red Modifier Reagent||1 vial||1 vial||1 vial|
|Texas Red Quencher Reagent||1 vial||1 vial||1 vial|
Our Abpromise guarantee covers the use of ab195225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent concentration.|
ab195225 has not yet been referenced specifically in any publications.