Overview

  • Product nameAnti-TFAP2E antibody
    See all TFAP2E primary antibodies
  • Description
    Rabbit polyclonal to TFAP2E
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow
  • Immunogen

    A region within synthetic peptide: GGPAICAALT AFQNYLLESL KGLDKMFLSS VGSGHGETKA SEKDAKHRK, corresponding to C terminal amino acids 394-442 of Human TFAP2E

  • Positive control
    • Transfected 293T nuclear lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab56295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).Can be blocked with TFAP2E peptide (ab175182). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionSequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-epsilon may play a role in the development of the CNS and in cartilage differentiation.
  • Tissue specificityExpressed in skin, primary keratinocytes, immortalized keratinocytes, and HeLa cell line.
  • Sequence similaritiesBelongs to the AP-2 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating enhancer binding protein 2 epsilon antibody
    • Activating enhancer-binding protein 2-epsilon antibody
    • Adaptor related protein complex 2 epsilon subunit antibody
    • AP 2E antibody
    • AP2 epsilon antibody
    • AP2-epsilon antibody
    • AP2E antibody
    • AP2E_HUMAN antibody
    • MGC49007 antibody
    • TFAP 2E antibody
    • TFAP2E antibody
    • Transcription factor AP 2 epsilon (activating enhancer binding protein 2 epsilon) antibody
    • Transcription factor AP 2 epsilon antibody
    • Transcription factor AP-2-epsilon antibody
    see all

Anti-TFAP2E antibody images

  • Anti-TFAP2E antibody (ab56295) at 1.25 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

References for Anti-TFAP2E antibody (ab56295)

ab56295 has not yet been referenced specifically in any publications.

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I've been in touch with the lab about ab56295 and our other TFAP2E antibody, ab89965, and neither of these has been tested for reactivity against the other family members, so we are unable to gua...

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