Overview

  • Product name
  • Description
    Rabbit polyclonal to TFAP4
  • Specificity
    ab28512 recognises Transcription factor AP-4 (TFAP4).
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    A region within a synthetic peptide: EEEQRRAVIV KPVRSCPEAP TSDTASDSEA SDSDAMDQSR EEPSGDGELP, corresponding to C terminal amino acids 289-338 of Human TFAP4

  • Positive control
    • Jurkat Cell Lysate. Human brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab28512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/321500.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Transcription factor that activates both viral and cellular genes by binding to the symmetrical DNA sequence 5'-CAGCTG-3'.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating enhancer binding protein 4 antibody
    • Activating enhancer-binding protein 4 antibody
    • AP 4 antibody
    • AP4 antibody
    • bHLHc41 antibody
    • Class C basic helix-loop-helix protein 41 antibody
    • TFAP 4 antibody
    • TFAP4 antibody
    • TFAP4 protein antibody
    • TFAP4_HUMAN antibody
    • Transcription factor AP 4 antibody
    • Transcription factor AP-4 antibody
    • Transcription factor AP4 antibody
    see all

Images

  • ab28512 at 4.0-8.0 ug/ml, staining TFAP4 in human brain cells (neural cells) by immunohistochmistry, paraffin embedded tissue. Cells with positive label indicated with arrows.
  • Anti-TFAP4 antibody (ab28512) at 1.25 µg/ml + Jurkat Cell Lysate

    Secondary
    Rabbit Anti-TFAP4 at 1/50000 dilution

    Predicted band size : 39 kDa

References

This product has been referenced in:
  • Ge Z  et al. Molecular mechanism of activating protein-4 regulated growth of hepatocellular carcinoma. Tumour Biol N/A:N/A (2014). Read more (PubMed: 25195135) »
  • Dahabieh MS  et al. Identification and functional analysis of a second RBF-2 binding site within the HIV-1 promoter. Virology 418:57-66 (2011). Read more (PubMed: 21813151) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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