The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. PubMed: 21113058
Use at an assay dependent dilution. Can be blocked with Human TFB2M peptide (ab23228). Preliminary experiments gave an approx 40kDa band in Human Brain lysate at 0.3 ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 46kDda according to NP_071761
FunctionS-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.