Overview

  • Product nameAnti-TFB2M antibody
    See all TFB2M primary antibodies
  • Description
    Rabbit polyclonal to TFB2M
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse
  • Immunogen

    A region within synthetic peptide: LTQALLEAGA KVVALESDKT FIPHLESLGK NLDGKLRVIH CDFFKLDPRS, corresponding to N terminal amino acids 109-158 of Human TFB2M

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionS-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • N''-adenosyl(rRNA) dimethyltransferase 2 antibody
    • S adenosylmethionine 6 N', N' adenosyl(rRNA) dimethyltransferase 2 antibody
    • Dimethyladenosine transferase 2 antibody
    • dimethyladenosine transferase 2 mitochondrial antibody
    • FLJ22661 antibody
    • FLJ23182 antibody
    • h mtTFB antibody
    • h mtTFB2 antibody
    • h-mtTFB antibody
    • h-mtTFB2 antibody
    • HCV NS5A transactivated protein 5 antibody
    • HCV NS5A-transactivated protein 5 antibody
    • hepatitis C virus NS5A transactivated protein 5 antibody
    • Hepatitis C virus NS5A-transactivated protein 5 antibody
    • Hkp1 antibody
    • hTFB2M antibody
    • Mitochondrial 12S rRNA dimethylase 2 antibody
    • mitochondrial antibody
    • Mitochondrial dimethyladenosine transferase 2 antibody
    • Mitochondrial transcription factor B2 antibody
    • mtTFB antibody
    • mtTFB2 antibody
    • NS5ATP5 antibody
    • S-adenosylmethionine-6-N'' antibody
    • TFB2M antibody
    • TFB2M_HUMAN antibody
    • Transcription Factor B2 antibody
    • Transcription factor B2 mitochondrial antibody
    see all

Anti-TFB2M antibody images

  • Anti-TFB2M antibody (ab66014) at 1.25 µg/ml + transfected 293T cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 50,000 - 100,000 dilution

    Predicted band size : 45 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling TFB2M with ab66014 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnification: 400X.

References for Anti-TFB2M antibody (ab66014)

This product has been referenced in:
  • Pohjoismäki JL  et al. Oxidative stress during mitochondrial biogenesis compromises mtDNA integrity in growing hearts and induces a global DNA repair response. Nucleic Acids Res 40:6595-607 (2012). WB ; Rat . Read more (PubMed: 22508755) »
  • Karamanlidis G  et al. Defective DNA replication impairs mitochondrial biogenesis in human failing hearts. Circ Res 106:1541-8 (2010). Read more (PubMed: 20339121) »

See all 2 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"