Overview

  • Product nameAnti-TFB2M antibody
    See all TFB2M primary antibodies
  • Description
    Mouse monoclonal to TFB2M
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: YLIQMIPRQN LFTKNLTPMN YNIFFHLLKH CFGRRSATVI DHLRSLTPLD ARDILMQIGK QEDEKVVNMH PQDFKTLFET IERSKDCAYK WLYDETLEDR, corresponding to amino acids 297-396 of Human TFB2M (NP_071761) with a proprietary tag.

  • Positive control
    • Partial recombinant tagged TFB2M protein. Human kidney tissue lysate. HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab88187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionS-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • N''-adenosyl(rRNA) dimethyltransferase 2 antibody
    • S adenosylmethionine 6 N', N' adenosyl(rRNA) dimethyltransferase 2 antibody
    • Dimethyladenosine transferase 2 antibody
    • dimethyladenosine transferase 2 mitochondrial antibody
    • FLJ22661 antibody
    • FLJ23182 antibody
    • h mtTFB antibody
    • h mtTFB2 antibody
    • h-mtTFB antibody
    • h-mtTFB2 antibody
    • HCV NS5A transactivated protein 5 antibody
    • HCV NS5A-transactivated protein 5 antibody
    • hepatitis C virus NS5A transactivated protein 5 antibody
    • Hepatitis C virus NS5A-transactivated protein 5 antibody
    • Hkp1 antibody
    • hTFB2M antibody
    • Mitochondrial 12S rRNA dimethylase 2 antibody
    • mitochondrial antibody
    • Mitochondrial dimethyladenosine transferase 2 antibody
    • Mitochondrial transcription factor B2 antibody
    • mtTFB antibody
    • mtTFB2 antibody
    • NS5ATP5 antibody
    • S-adenosylmethionine-6-N'' antibody
    • TFB2M antibody
    • TFB2M_HUMAN antibody
    • Transcription Factor B2 antibody
    • Transcription factor B2 mitochondrial antibody
    see all

Anti-TFB2M antibody images

  • Anti-TFB2M antibody (ab88187) at 1 µg/ml + Partial recombinant tagged TFB2M protein (Immunogen) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 45 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-TFB2M antibody (ab88187) at 1 µg/ml + human kidney tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 45 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Anti-TFB2M antibody (ab88187) at 1 µg/ml + HeLa cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 45 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-TFB2M antibody (ab88187)

ab88187 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab88187.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"