Overview

  • Product nameAnti-TFE3 antibody
    See all TFE3 primary antibodies
  • Description
    Chicken polyclonal to TFE3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 432-541 of Human TFE3

  • Positive control
    • E. coli-derived TFE3 fusion protein

Properties

Applications

Our Abpromise guarantee covers the use of ab36642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).

Target

  • FunctionTranscription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
  • Tissue specificityUbiquitous in fetal and adult tissues.
  • Involvement in diseaseNote=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
    Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
  • Sequence similaritiesBelongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Sumoylated; does not affect dimerization with MITF.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLH e33 antibody
    • bHLHe33 antibody
    • Class E basic helix-loop-helix protein 33 antibody
    • RCCP 2 antibody
    • RCCP2 antibody
    • RCCX1 antibody
    • Renal cell carcinoma, papillary antibody
    • Tcfe 3 antibody
    • Tcfe3 antibody
    • TFE 3 antibody
    • Tfe3 antibody
    • TFE3_HUMAN antibody
    • TFEA antibody
    • Transcription factor binding to IGHM enhancer 3 antibody
    • Transcription factor E family, member A antibody
    • Transcription factor E3 antibody
    • Transcription factor for IgH enhancer antibody
    • Transcription factor for immunoglobulin heavy chain enhancer 3 antibody
    see all

Anti-TFE3 antibody images

  • Anti-TFE3 antibody (ab36642) at 1/2000 dilution + E. coli-derived TFE3 fusion protein at 2.5 µg

    Secondary
    Goat anti-IgY-HRP at 1/1000 dilution

    Predicted band size : 80 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-TFE3 antibody (ab36642)

ab36642 has not yet been referenced specifically in any publications.

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