Overview

  • Product name
  • Description
    Rabbit polyclonal to TFE3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TFE3.

    (Peptide available as ab109924.)

  • Positive control
    • This antibody gave a positive signal in A549 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).

Target

  • Function
    Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
  • Tissue specificity
    Ubiquitous in fetal and adult tissues.
  • Involvement in disease
    Note=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
    Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
  • Sequence similarities
    Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Sumoylated; does not affect dimerization with MITF.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLH e33 antibody
    • bHLHe33 antibody
    • Class E basic helix-loop-helix protein 33 antibody
    • RCCP 2 antibody
    • RCCP2 antibody
    • RCCX1 antibody
    • Renal cell carcinoma, papillary antibody
    • Tcfe 3 antibody
    • Tcfe3 antibody
    • TFE 3 antibody
    • Tfe3 antibody
    • TFE3_HUMAN antibody
    • TFEA antibody
    • Transcription factor binding to IGHM enhancer 3 antibody
    • Transcription factor E family, member A antibody
    • Transcription factor E3 antibody
    • Transcription factor for IgH enhancer antibody
    • Transcription factor for immunoglobulin heavy chain enhancer 3 antibody
    see all

Images

  • All lanes : Anti-TFE3 antibody (ab86127) at 1 µg/ml

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 62 kDa


    Exposure time : 12 minutes

References

ab86127 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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