Overview

  • Product name
    Anti-TFE3 antibody - N-terminal
    See all TFE3 primary antibodies
  • Description
    Rabbit polyclonal to TFE3 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TFE3 aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P19532

  • Positive control
    • 293 cell lysate; mouse lung lysate; Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab173928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 62 kDa.
ICC/IF 1/10 - 1/50.

Target

  • Function
    Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
  • Tissue specificity
    Ubiquitous in fetal and adult tissues.
  • Involvement in disease
    Note=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
    Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
  • Sequence similarities
    Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Sumoylated; does not affect dimerization with MITF.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLH e33 antibody
    • bHLHe33 antibody
    • Class E basic helix-loop-helix protein 33 antibody
    • RCCP 2 antibody
    • RCCP2 antibody
    • RCCX1 antibody
    • Renal cell carcinoma, papillary antibody
    • Tcfe 3 antibody
    • Tcfe3 antibody
    • TFE 3 antibody
    • Tfe3 antibody
    • TFE3_HUMAN antibody
    • TFEA antibody
    • Transcription factor binding to IGHM enhancer 3 antibody
    • Transcription factor E family, member A antibody
    • Transcription factor E3 antibody
    • Transcription factor for IgH enhancer antibody
    • Transcription factor for immunoglobulin heavy chain enhancer 3 antibody
    see all

Images

  • Anti-TFE3 antibody - N-terminal (ab173928) at 1/100 dilution + Mouse lung lysate at 35 µg

    Predicted band size: 62 kDa

  • Anti-TFE3 antibody - N-terminal (ab173928) at 1/100 dilution + 293 cell lysate at 35 µg

    Predicted band size: 62 kDa

  • Confocal immunofluorescent analysis of Hela cells labeling TFE3 with ab173928. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab173928 (1/25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1/400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). 

References

ab173928 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab173928.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up