Anti-TFEB antibody (ab82143)




Our Abpromise guarantee covers the use of ab82143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:32,000.
    WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 53 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.
    • Sequence similaritiesBelongs to the MiT/TFE family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • DomainThe leucin zipper region is essential for homo- or heterodimerization and high-affinity DNA binding. DNA binding is mediated by the basic region.
    • Post-translational
      Sumoylated; does not affect dimerization with MITF.
    • Cellular localizationCytoplasm. Nucleus. Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha TFEB antibody
      • AlphaTFEB antibody
      • bHLHe35 antibody
      • Class E basic helix-loop-helix protein 35 antibody
      • T cell transcription factor EB antibody
      • TCFEB antibody
      • TFEB antibody
      • TFEB_HUMAN antibody
      • Transcription factor EB antibody
      see all

    Anti-TFEB antibody images

    • Anti-TFEB antibody (ab82143) at 1 µg/ml + human lung lysate in RIPA buffer at 35 µg
      developed using the ECL technique

      Predicted band size : 53 kDa
      Observed band size : 53 kDa

    References for Anti-TFEB antibody (ab82143)

    ab82143 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (mouse Schwann cell)
    Loading amount 20 µg
    Specification mouse Schwann cell
    Treatment starvation 8 hours
    Gel Running Conditions Reduced Denaturing (12.5%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Ms. Jessica Nicks

    Verified customer

    Submitted Jan 29 2013

    I see that you mentioned in the additional notes that there was a very faint band at 43kDa. I just wanted to inform you that the expected band size for TFEB is 53kDa. Did you see a positive band around the 53dDa size?
    I am sorry to hear that y...

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    Thank you for your phone call.

    I have arranged for the free of charge replacement with the human lung tissue lysate ab30281. The order number is xxx and you should receive it tomorrow.

    Please let me know if you have further questions....

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