Our Abpromise guarantee covers the use of ab2636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
|ChIP||Use at an assay dependent concentration. PubMed: 17085443|
|IHC-P||Use a concentration of 2 - 4 µg/ml.|
ab2636 (2µg/ml) staining of paraffin embedded Human Kidney cancer shows strong nuclear staining of invading cells following Steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.