Based on sequence homology, TFEB isoforms 1 and 2 (NP_009093.1 and NP_001161299.2) with MW of 52.9 and 54.2 kDa respectively can be detected by this antibody
Principal Names - TFEB; transcription factor EB; TCFEB; T-cell transcription factor EB Official Gene Symbol - TFEB GenBank Accession Number – NP_009093 LocusLink ID - 7942 (human); 21425 (mouse)
Our Abpromise guarantee covers the use of ab2636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1.5 µg/ml.
Detects band(s) of approximately 53 kDa. Predicted molecular weights of TFEB isoforms 1 and 2 are 52.9 and 54.2 kDa respectively.
|ChIP||Use at an assay dependent concentration. PubMed: 17085443|
|IHC-P||Use a concentration of 2 - 4 µg/ml.|
ab2636 staining (1µg/ml) of NIH-3T3 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab2636 staining (1µg/ml) of NIH-3T3 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ab2636 (2µg/ml) staining of paraffin embedded Human Kidney cancer shows strong nuclear staining of invading cells following Steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.