The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 46 kDa).
Use at an assay dependent dilution.
RelevanceThe basal transcription factor TFIIF binds with RNA polymerase II and takes part in the transcription initiation reaction and the elongation reaction. Budding yeast TFIIF is composed of three subunits, Tfg1p (735aa), Tfg2p (400aa) and Taf14p (244aa).