The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 135 kDa).
Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.
Involvement in disease
Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Belongs to the TFII-I family. Contains 6 GTF2I-like repeats.
Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation. Sumoylated.
Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm.
Bruton tyrosine kinase associated protein 135 antibody
Bruton tyrosine kinase-associated protein 135 antibody
BTK associated protein 135 antibody
BTK associated protein 135kD antibody
BTK associated protein antibody
BTK-associated protein 135 antibody
BTKAP 1 antibody
General transcription factor II i antibody
General transcription factor II-I antibody
General transcription factor IIi antibody
GTF 2I antibody
GTFII I antibody
IB 291 antibody
SRF Phox 1 interacting protein antibody
SRF Phox1 interacting protein antibody
SRF-Phox1-interacting protein antibody
Transcription factor II I antibody
WBSCR 6 antibody
Williams Beuren syndrome chromosome region 6 antibody
Williams Beuren syndrome chromosome region 6 protein antibody
Williams-Beuren syndrome chromosomal region 6 protein antibody
Western blot - TFII I antibody (ab10464)
Predicted band size : 135 kDa
ab10464 detection of human TFII I by Western blot. Samples: Whole cell lysate (100 µg) from HEK293 cells treated with TFII-I siRNA (+) or untreated (-). Antibody: ab10464 used at 0.2 µg/ml. Detection: Chemiluminescence.
Narla ST et al. Activation of developmental nuclear fibroblast growth factor receptor 1 signaling and neurogenesis in adult brain by a7 nicotinic receptor agonist. Stem Cells Transl Med2:776-88 (2013).
Read more (PubMed: 24014683) »
Crusselle-Davis VJ et al. Recruitment of coregulator complexes to the beta-globin gene locus by TFII-I and upstream stimulatory factor. FEBS J274:6065-73 (2007).
Read more (PubMed: 17970752) »