Anti-TFII I (phospho Y248) antibody (ab63344)
Key features and details
- Rabbit polyclonal to TFII I (phospho Y248)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TFII I (phospho Y248) antibody
See all TFII I primary antibodies -
Description
Rabbit polyclonal to TFII I (phospho Y248) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human TFII I aa 200-300 (phospho Y248).
Database link: P78347 -
Positive control
- Extracts from LOVO cells
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa). |
Target
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Function
Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation. -
Tissue specificity
Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues. -
Involvement in disease
Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
Sequence similarities
Belongs to the TFII-I family.
Contains 6 GTF2I-like repeats. -
Post-translational
modificationsTransiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
Sumoylated. -
Cellular localization
Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2969 Human
- Entrez Gene: 14886 Mouse
- Entrez Gene: 353256 Rat
- Omim: 601679 Human
- SwissProt: P78347 Human
- SwissProt: Q9ESZ8 Mouse
- SwissProt: Q5U2Y1 Rat
- Unigene: 647041 Human
see all -
Alternative names
- BAP 135 antibody
- BAP-135 antibody
- BAP135 antibody
see all
Images
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All lanes : Anti-TFII I (phospho Y248) antibody (ab63344) at 1/500 dilution
Lane 1 : extracts from LOVO cells, without immunising peptide
Lane 2 : extracts from LOVO cells, with immunising peptide
Predicted band size: 112 kDa
Observed band size: 112 kDa
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
The amount of cell extracts loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab63344 has been referenced in 1 publication.
- Gui F et al. A non-covalent inhibitor XMU-MP-3 overrides ibrutinib-resistant BtkC481S mutation in B-cell malignancies. Br J Pharmacol 176:4491-4509 (2019). PubMed: 31364164