This antibody gave a positive result in ELISA against the immunizing peptide (ab26407). It gave a negative result in ELISA against the non-modified equivalent peptide (ab25428). This indicates that it is specific for the modified peptide.
See figure below.
IF/ICC: See figure below.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Belongs to the TFIIB family. Contains 1 TFIIB-type zinc finger.
This antibody gave a positive result in ELISA against the immunising peptide (ab26407) and a negative result using the non-modified equivalent peptide (ab25428). This indicates that it is specific for the modified peptide.
Red line: ELISA against immunising peptide ab26407
Green line: ELISA against non-modified peptide ab25428
ICC/IF image of ab5210 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab5210, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and Mcf7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.