Overview

  • Product nameAnti-TFIIB antibody
    See all TFIIB primary antibodies
  • Description
    Mouse polyclonal to TFIIB
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Plasmodium falciparum
  • Immunogen

    Recombinant fusion protein: TTQKKTTIKN VIKTKNLKRE DMETENEQNE AQPNNEKNEA QPNNEKNKAP PNDEKNEAPS NEENVVQDLN KDELSDNLEN NPVKNFDTDF ENKNDGEEKY, corresponding to amino acids 195-294 of Plasmodium falciparum TFIIB

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 35 kDa).

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionGeneral factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
  • Sequence similaritiesBelongs to the TFIIB family.
    Contains 1 TFIIB-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Alternative names
    • General transcription factor IIB antibody
    • General transcription factor TFIIB antibody
    • gtf2b antibody
    • RNA polymerase II transcription factor IIB antibody
    • S300 II antibody
    • S300-II antibody
    • TF IIB antibody
    • TF2B antibody
    • TF2B_HUMAN antibody
    • TFIIB antibody
    • Transcription initiation factor IIB antibody
    see all

Anti-TFIIB antibody images

  • All lanes : Anti-TFIIB antibody (ab52730) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a recombinant fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen (tagged recombinant fusion protein)

    Secondary
    anti-mouse IgG + IgM, horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 35 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

References for Anti-TFIIB antibody (ab52730)

ab52730 has not yet been referenced specifically in any publications.

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