Overview

  • Product nameAnti-TFIIB antibody
    See all TFIIB primary antibodies
  • Description
    Rabbit polyclonal to TFIIB
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 252-301 (SVAAAAIYMA SQASAEKRTQ KEIGDIAGVA DVTIRQSYRL IYPRAPDLFP) of Human TFIIB (NP_001505).

  • Positive control
    • Huma placenta lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

Anti-TFIIB antibody images

  • Anti-TFIIB antibody (ab82989) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TFIIB antibody (ab82989)

ab82989 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"