• Product nameAnti-TFIIB antibody [IIB8]
    See all TFIIB primary antibodies
  • Description
    Mouse monoclonal [IIB8] to TFIIB
  • Tested applicationsSuitable for: Functional Studies, EMSA, ELISA, IP, Purification, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Partially purified recombinant human transcription factor IIB (TFIIB, Promega Corp.).

  • EpitopeAmino acids 52-105 of human and mouse TFIIB.



Our Abpromise guarantee covers the use of ab819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution. This antibody can also immuno-deplete TFIIB from cell nuclear extracts and inhibit transcription of TFIIB from cell nuclear extracts.
EMSA Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
Purification Use at an assay dependent dilution. This antibody is polyol-responsive and can be used for gentle purification of TFIIB.
WB Use at an assay dependent dilution. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).


  • FunctionGeneral factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
  • Sequence similaritiesBelongs to the TFIIB family.
    Contains 1 TFIIB-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • General transcription factor IIB antibody
    • General transcription factor TFIIB antibody
    • gtf2b antibody
    • RNA polymerase II transcription factor IIB antibody
    • S300 II antibody
    • S300-II antibody
    • TF IIB antibody
    • TF2B antibody
    • TF2B_HUMAN antibody
    • TFIIB antibody
    • Transcription initiation factor IIB antibody
    see all

References for Anti-TFIIB antibody [IIB8] (ab819)

This product has been referenced in:
  • Paes de Faria J & Fernandes L Protection against oxidative stress through SUA7/TFIIB regulation in Saccharomyces cerevisiae. Free Radic Biol Med 41:1684-93 (2006). WB ; Saccharomyces cerevisiae . Read more (PubMed: 17145557) »
  • Thompson NE  et al. Accessibility of epitopes on human transcription factor IIB in the native protein and in a complex with DNA. J Biol Chem 270:4735-40 (1995). Read more (PubMed: 7533165) »

See all 3 Publications for this product

Product Wall

Thank you for getting back to us and sharing your results. We have looked at our database but did not receive any similar complaint so far. We are very sorry to inform you that we have sold out all the vials from Lot: 12091. However, we have new ba...

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Thank you for your message. We have had no other complaints regarding this antibody or about other batches. I'm happy that you have had success with ab819 in the past and would like to reassure you that our policy is that if you happened to have proble...

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