TFIIB overexpression 293T lysate (whole cell) (ab94156)

Overview

  • Product nameTFIIB overexpression 293T lysate (whole cell)
    See all TFIIB lysates
  • Description
    TFIIB overexpression lysate
  • General notesab94156 is a 293T cell transfected lysate in which Human TFIIB has been transiently over-expressed using a pCMV-TFIIB plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. Similarity: Belongs to the TFIIB family. Contains 1 TFIIB-type zinc finger.

Applications

Our Abpromise guarantee covers the use of ab94156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

TFIIB overexpression 293T lysate (whole cell) images

  • ab94156 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-TFIIB antibody (ab88532) at 1/500 dilution

    Lane 1 : TFIIB overexpression 293T lysate (whole cell) (ab94156)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for TFIIB overexpression 293T lysate (whole cell) (ab94156)

ab94156 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"