The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionRecruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Sequence similaritiesBelongs to the TFIIE alpha subunit family. Contains 1 HTH TFE/IIEalpha-type domain.
ab99418 at 0.4 µg/ml detecting TFIIE alpha in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 3 second exposure.
For immunoprecipitation, ab99416 and ab99418 were used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
Lane 1; IP using ab99416
Lane 2; IP using ab99418 a rabbit anti-TFIIE alpha antibody which recognizes a downstream epitope.
Lane 3; IP using control IgG.
References for Anti-TFIIE alpha antibody (ab99416)
has not yet been referenced specifically in any publications.
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