• Product nameAnti-TFIIE alpha antibody
    See all TFIIE alpha primary antibodies
  • Description
    Rabbit polyclonal to TFIIE alpha
  • Tested applicationsSuitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human TFIIE alpha aa 100-150. The exact sequence is proprietary.
    Database link: NP_005504.2

  • Positive control
    • HeLa cell lysate
  • General notesAntibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.



Our Abpromise guarantee covers the use of ab99416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionRecruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
    • Sequence similaritiesBelongs to the TFIIE alpha subunit family.
      Contains 1 HTH TFE/IIEalpha-type domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FE antibody
      • General transcription factor IIE 56 kDa subunit antibody
      • General transcription factor IIE subunit 1 antibody
      • General transcription factor IIE, polypeptide 1, alpha 56kDa antibody
      • Gtf2e1 antibody
      • MAL7P1.86 antibody
      • T2EA_HUMAN antibody
      • TF2E1 antibody
      • TFIIE A antibody
      • TFIIE alpha antibody
      • TFIIE-alpha antibody
      • Transcription initiation factor IIE alpha subunit antibody
      • Transcription initiation factor iie alpha subunit putative antibody
      • Transcription initiation factor IIE subunit alpha antibody
      see all

    Anti-TFIIE alpha antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling TFIIE alpha with ab99416 at 1/1000 (1µg/ml). Detection: DAB.
    • ab99418 at 0.4 µg/ml detecting TFIIE alpha in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 3 second exposure.
      For immunoprecipitation, ab99416 and ab99418 were used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
      Lane 1; IP using ab99416
      Lane 2; IP using ab99418 a rabbit anti-TFIIE alpha antibody which recognizes a downstream epitope.
      Lane 3; IP using control IgG.

    References for Anti-TFIIE alpha antibody (ab99416)

    ab99416 has not yet been referenced specifically in any publications.

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