Overview

  • Product nameAnti-TFIIE alpha antibody
    See all TFIIE alpha primary antibodies
  • Description
    Rabbit polyclonal to TFIIE alpha
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Other Immunogen Type within Human TFIIE alpha aa 389-439. The exact sequence is proprietary.
    Database link: NP_005504.2

  • Positive control
    • HeLa whole cell lysate, 293T whole cell lysate and Mouse NIH3T3 whole cell lysate.
  • General notesAntibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties

Applications

Our Abpromise guarantee covers the use of ab99418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-TFIIE alpha antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling TFIIE alpha with ab99418 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TFIIE alpha antibody (ab99418) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 49 kDa


    Exposure time : 30 seconds
  • ab99418 at 0.4 µg/ml detecting TFIIE alpha in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 3 second exposure.
    For immunoprecipitation, ab99418 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99416, a rabbit anti-TFIIE alpha antibody which recognizes an upstream epitope
    Lane 2; IP using ab99418.
    Lane 3; IP using control IgG.

References for Anti-TFIIE alpha antibody (ab99418)

ab99418 has not yet been referenced specifically in any publications.

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