Anti-TFIIE alpha antibody - ChIP Grade (ab28177)

Overview

  • Product nameAnti-TFIIE alpha antibody - ChIP Grade
    See all TFIIE alpha primary antibodies
  • Description
    Rabbit polyclonal to TFIIE alpha - ChIP Grade
  • Specificityab28177 recognises TFIIE alpha.
  • Tested applicationsSuitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human): TFIIE alpha

  • Positive control
    • HeLa nuclear extract

Properties

Applications

Our Abpromise guarantee covers the use of ab28177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
ChIP Use 5-10 µg for 25 µg of chromatin.

Target

  • FunctionRecruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
  • Sequence similaritiesBelongs to the TFIIE alpha subunit family.
    Contains 1 HTH TFE/IIEalpha-type domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FE antibody
    • General transcription factor IIE 56 kDa subunit antibody
    • General transcription factor IIE subunit 1 antibody
    • General transcription factor IIE, polypeptide 1, alpha 56kDa antibody
    • Gtf2e1 antibody
    • MAL7P1.86 antibody
    • T2EA_HUMAN antibody
    • TF2E1 antibody
    • TFIIE A antibody
    • TFIIE alpha antibody
    • TFIIE-alpha antibody
    • Transcription initiation factor IIE alpha subunit antibody
    • Transcription initiation factor iie alpha subunit putative antibody
    • Transcription initiation factor IIE subunit alpha antibody
    see all

Anti-TFIIE alpha antibody - ChIP Grade images

  • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 8µg of  ab28177 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).

References for Anti-TFIIE alpha antibody - ChIP Grade (ab28177)

ab28177 has not yet been referenced specifically in any publications.

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