The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
1/50 - 1/100.
FunctionRecruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Sequence similaritiesBelongs to the TFIIE beta subunit family. Contains 1 TFIIE beta DNA-binding domain.