Overview

  • Product nameAnti-TFIIE beta antibody
    See all TFIIE beta primary antibodies
  • Description
    Rabbit polyclonal to TFIIE beta
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 242-291 (ISSMQESGPK KVAPIQRRKK PASQKKRRFK THNEHLAGVL KDYSDITSSK) of Human TFIIE beta (NP_002086).

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionRecruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
  • Sequence similaritiesBelongs to the TFIIE beta subunit family.
    Contains 1 TFIIE beta DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FE antibody
    • General transcription factor IIE subunit 2 antibody
    • General transcription factor IIE, polypeptide 2, beta 34kDa antibody
    • gtf2e2 antibody
    • T2EB_HUMAN antibody
    • TF2E 2 antibody
    • TF2E2 antibody
    • TFIIE B antibody
    • TFIIE-beta antibody
    • Transcription initiation factor IIE beta subunit antibody
    • Transcription initiation factor IIE subunit beta antibody
    see all

Anti-TFIIE beta antibody images

  • Anti-TFIIE beta antibody (ab83484) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TFIIE beta antibody (ab83484)

ab83484 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83484.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"