Overview

  • Product nameAnti-TFIIF antibody
    See all TFIIF primary antibodies
  • Description
    Rabbit polyclonal to TFIIF
  • SpecificityThis antibody reacts with Transcription initiation factor IIF beta subunit (GTF2F2), polypeptide 2, 30kDa.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: QLDKVVTTNY KPVANHQYNI EYERKKKEDG KRARADKQHV LDMLFSAFEK, corresponding to internal sequence amino acids 144-193 of Human TFIIF

  • Positive control
    • HepG2 cell lysate, Human brain tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5625e+006.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • RelevanceThe RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • ATP dependent helicase GTF2F2 antibody
    • BTF 4 antibody
    • BTF4 antibody
    • General transcription factor IIF polypeptide 1 antibody
    • General transcription factor IIF polypeptide 1 74 kDa subunit protein antibody
    • General transcription factor IIF polypeptide 1 74kDa antibody
    • General transcription factor IIF polypeptide 2 30kDa antibody
    • General Transcription Factor IIF Polypeptide 2 antibody
    • GTF2F1 antibody
    • GTF2F1 protein antibody
    • GTF2F2 antibody
    • RAP 30 antibody
    • RAP 74 antibody
    • RAP30 antibody
    • RAP74 antibody
    • TF2F 1 antibody
    • TF2F 2 antibody
    • TF2F1 antibody
    • TF2F2 antibody
    • TFIIF alpha antibody
    • TFIIF beta antibody
    • Transcription initiation factor IIF alpha subunit antibody
    • Transcription Initiation Factor IIF beta Subunit antibody
    • Transcription initiation factor IIF subunit beta antibody
    • Transcription initiation factor RAP30 antibody
    • Transcription initiation factor RAP74 antibody
    see all

Anti-TFIIF antibody images

  • Anti-TFIIF antibody (ab49154) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG 1/50,000 - 1/100,000

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling TFIIF with ab49154 at 4-8µg/ml. Arrows indicate positively labelled neural cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spermatophore tissue labelling TFIIF with ab49154 at 4-8µg/ml. Arrows indicate postively labelled epithelial cells. Magnification: 400X.

References for Anti-TFIIF antibody (ab49154)

ab49154 has not yet been referenced specifically in any publications.

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