Overview

  • Product nameAnti-TFIIF antibody - ChIP Grade
    See all TFIIF primary antibodies
  • Description
    Rabbit polyclonal to TFIIF - ChIP Grade
  • SpecificityThis antibody is specific to the RAP30 subunit of TFIIF.
  • Tested applicationsSuitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein - RAP30 subunit of the TFIIF complex.

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab28180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 84 kDa.
ChIP Use 5-10 µg for 25 µg of chromatin.

Target

  • RelevanceThe RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • ATP dependent helicase GTF2F2 antibody
    • BTF 4 antibody
    • BTF4 antibody
    • General transcription factor IIF polypeptide 1 antibody
    • General transcription factor IIF polypeptide 1 74 kDa subunit protein antibody
    • General transcription factor IIF polypeptide 1 74kDa antibody
    • General transcription factor IIF polypeptide 2 30kDa antibody
    • General Transcription Factor IIF Polypeptide 2 antibody
    • GTF2F1 antibody
    • GTF2F1 protein antibody
    • GTF2F2 antibody
    • RAP 30 antibody
    • RAP 74 antibody
    • RAP30 antibody
    • RAP74 antibody
    • TF2F 1 antibody
    • TF2F 2 antibody
    • TF2F1 antibody
    • TF2F2 antibody
    • TFIIF alpha antibody
    • TFIIF beta antibody
    • Transcription initiation factor IIF alpha subunit antibody
    • Transcription Initiation Factor IIF beta Subunit antibody
    • Transcription initiation factor IIF subunit beta antibody
    • Transcription initiation factor RAP30 antibody
    • Transcription initiation factor RAP74 antibody
    see all

Anti-TFIIF antibody - ChIP Grade images

  • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The  ChIP was performed with 25µg of chromatin, 8µg of  ab28180 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.


References for Anti-TFIIF antibody - ChIP Grade (ab28180)

This product has been referenced in:
  • Hogel M  et al. Promoters are differentially sensitive to N-terminal mutant huntingtin-mediated transcriptional repression. PLoS One 7:e41152 (2012). Read more (PubMed: 22815947) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"