Overview

  • Product name
    Anti-TFIIIC / GTF3C1 antibody
    See all TFIIIC / GTF3C1 primary antibodies
  • Description
    Rabbit polyclonal to TFIIIC / GTF3C1
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 2059 and 2109 of human TFIIIC/ GTF3C1 protein (NP_001511.2)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab75251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 238 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance
      The transcription factor IIIC (TFIIIC) is a multi-subunit DNA-binding factor required for promoter recognition and TFIIIB assembly on tRNA genes transcribed by RNA polymerase III.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • General transcription factor 3C polypeptide 1 antibody
      • GTF3C1 antibody
      • TF3C-alpha antibody
      • TFIIIC 220 kDa subunit antibody
      • TFIIIC box B-binding subunit antibody
      • TFIIIC220 antibody
      • Transcription factor IIIC-subunit alpha antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling TFIIIC / GTF3C1 with ab75251 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-TFIIIC / GTF3C1 antibody (ab75251) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 238 kDa
      Observed band size : 238 kDa


      Exposure time : 3 minutes

    References

    ab75251 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab75251.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up