The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:312500.
FunctionNecessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Tissue specificityTestis and ovary specific.
Sequence similaritiesBelongs to the TFS-II family. Contains 1 TFIIS central domain. Contains 1 TFIIS N-terminal domain. Contains 1 TFIIS-type zinc finger.
Transcription elongation factor A (SII) 2 antibody
Transcription elongation factor A 2 antibody
Transcription elongation factor A protein 2 antibody
Transcription elongation factor S II protein 2 antibody
Transcription elongation factor S-II protein 2 antibody
Transcription elongation factor TFIIS.1 antibody
Transcription elongation factor TFIIS.l antibody
Anti-TFIIS antibody images
Immunocytochemistry/ Immunofluorescence - Anti-TFIIS antibody (ab49352)This image is courtesy of an anonymous Abreview
ab49352 staining TFIIS in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 2% serum for 1 hour 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 1 x PBS) for 3 hour at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.