Overview

  • Product nameAnti-TFIIS antibody
    See all TFIIS primary antibodies
  • Description
    Mouse polyclonal to TFIIS
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human TFIIS protein (NP_003186.1).

  • Positive control
    • HeLa cells. HeLa cell lysate. TFIIS transfected 293T cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionNecessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
  • Tissue specificityTestis and ovary specific.
  • Sequence similaritiesBelongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Tcea2 antibody
    • TCEA2_HUMAN antibody
    • Testis specific S II antibody
    • Testis-specific S-II antibody
    • TFIIS antibody
    • Transcription elongation factor A (SII) 2 antibody
    • Transcription elongation factor A 2 antibody
    • Transcription elongation factor A protein 2 antibody
    • Transcription elongation factor S II protein 2 antibody
    • Transcription elongation factor S-II protein 2 antibody
    • Transcription elongation factor TFIIS.1 antibody
    • Transcription elongation factor TFIIS.l antibody
    see all

Anti-TFIIS antibody images

  • Anti-TFIIS antibody (ab88599) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • All lanes : Anti-TFIIS antibody (ab88599) at 1 µg/ml

    Lane 1 : TFIIS transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • ab88599, at 10 µg/ml, staining TFIIS in HeLa cells by immunofluorescence.

References for Anti-TFIIS antibody (ab88599)

ab88599 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Caenorhabditis elegans Cell (Intestinal Nuclei)
Specification Intestinal Nuclei
Fixative Paraformaldehyde
Permeabilization Yes - Sperm Salts Buffer, Triton X-100
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Submitted Jun 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"