Overview

  • Product name
  • Description
    Rabbit polyclonal to TFIISH
  • Specificity
    ab26141 recognises TCEA3.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide derived from a region between residues 228-277 of human TFIISH.

    .

  • Positive control
    • Jurkat cell lysate, human pancreatic cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab26141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
  • Sequence similarities
    Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Rhabdomyosarcoma antigen MU RMS 40.22 antibody
    • Tcea3 antibody
    • TCEA3_HUMAN antibody
    • TFIIS antibody
    • TFIIS.H antibody
    • Transcription elongation factor A (SII) 3 antibody
    • Transcription elongation factor A protein 3 antibody
    • Transcription elongation factor A3 antibody
    • Transcription elongation factor S-II protein 3 antibody
    • Transcription elongation factor TFIIS.h antibody
    see all

Images

  • Anti-TFIISH antibody (ab26141) at 0.5 µg/ml + Jurkat cell lysate

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Staining of parafin-embeded sections of human pancreas cells with ab26141 (4-8 ug/ml). Positive label on epithelial cells of pancreatic acinus indicated with arrows.

References

This product has been referenced in:
  • Abrahem A & Pelchat M Formation of an RNA polymerase II preinitiation complex on an RNA promoter derived from the hepatitis delta virus RNA genome. Nucleic Acids Res 36:5201-11 (2008). WB ; Human . Read more (PubMed: 18682525) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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