Overview

  • Product name
    Anti-TFIISH antibody [TCE5I551]
    See all TFIISH primary antibodies
  • Description
    Mouse monoclonal [TCE5I551] to TFIISH
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human TFIISH

  • Positive control
    • WB: Hela nuclear extract.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab50797 from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • Clonality
    Monoclonal
  • Clone number
    TCE5I551
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 44 kDa (predicted molecular weight: 39 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Function
    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
  • Sequence similarities
    Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Rhabdomyosarcoma antigen MU RMS 40.22 antibody
    • Tcea3 antibody
    • TCEA3_HUMAN antibody
    • TFIIS antibody
    • TFIIS.H antibody
    • Transcription elongation factor A (SII) 3 antibody
    • Transcription elongation factor A protein 3 antibody
    • Transcription elongation factor A3 antibody
    • Transcription elongation factor S-II protein 3 antibody
    • Transcription elongation factor TFIIS.h antibody
    see all

Images

  • Anti-TFIISH antibody [TCE5I551] (ab50797) at 1/100 dilution + HeLa nuclear extract at 20 µg/lane

    Secondary
    Mouse IgG antibody at 1/3000 dilution

    Predicted band size : 39 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)

References

ab50797 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab50797.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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