Overview

  • Product nameAnti-TFIISH antibody [TCE5I551]
    See all TFIISH primary antibodies
  • Description
    Mouse monoclonal [TCE5I551] to TFIISH
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human TFIISH

  • Positive control
    • WB: Hela nuclear extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab50797 from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • ClonalityMonoclonal
  • Clone numberTCE5I551
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 44 kDa (predicted molecular weight: 39 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionNecessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
  • Sequence similaritiesBelongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Tcea3 antibody
    • TCEA3_HUMAN antibody
    • TFIIS antibody
    • TFIIS.H antibody
    • Transcription elongation factor A (SII) 3 antibody
    • Transcription elongation factor A protein 3 antibody
    • Transcription elongation factor S-II protein 3 antibody
    • Transcription elongation factor TFIIS.h antibody
    see all

Anti-TFIISH antibody [TCE5I551] images

  • Anti-TFIISH antibody [TCE5I551] (ab50797) at 1/100 dilution + HeLa nuclear extract at 20 µg/lane

    Secondary
    Mouse IgG antibody at 1/3000 dilution

    Predicted band size : 39 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)

References for Anti-TFIISH antibody [TCE5I551] (ab50797)

ab50797 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab50797.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"