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Rabbit polyclonal to TFIP11
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human TFIP11 (NP_036275.1)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 97 kDa.
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix. May also be involved in pre-mRNA splicing.
Belongs to the TFP11/STIP family.
Contains 1 G-patch domain.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIP11 antibody (ab91520)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling TFIP11 with ab91520 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-TFIP11 antibody (ab91520)
All lanes : Anti-TFIP11 antibody (ab91520) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Developed using the ECL technique. Predicted band size: 97 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-TFIP11 antibody (ab91520)
Ab91520 at 1 µg/ml detecting TFIP11 by WB following IP. Lane 1; ab91520 at 3 µg/mg lysate precipitating TFIP11 from HeLa whole cell lysate. Lane 2; IP using an antibody to a downstream epitope of TFIP11. Lane 3; TFIP11 was not precipitated using control IgG. In each case 1 mg of whole cell lysate from HeLa cells was used for IP and 20% of IP was loaded.
Detection utilised ECL with a 10 second exposure.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"