Customer reviews and FAQs

Overview

  • Product name
    Anti-TFPI antibody
    See all TFPI primary antibodies
  • Description
    Rabbit polyclonal to TFPI
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TFPI.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab66543.)

  • Positive control
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat, A431, HEK293, HepG2, MCF7, SHSY-5Y and U2OS.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 35 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 24716194

Target

  • Function
    Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • Tissue specificity
    Mostly in endothelial cells.
  • Sequence similarities
    Contains 3 BPTI/Kunitz inhibitor domains.
  • Domain
    This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Anti convertin antibody
    • EPI antibody
    • Extrinsic pathway inhibitor antibody
    • LACI antibody
    • Lipoprotein associated coagulation inhibitor antibody
    • Lipoprotein-associated coagulation inhibitor antibody
    • TFI antibody
    • TFPI 1 antibody
    • TFPI antibody
    • TFPI1 antibody
    • TFPI1_HUMAN antibody
    • Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor) antibody
    • Tissue factor pathway inhibitor antibody
    see all

Anti-TFPI antibody images

  • Western blot - Anti-TFPI antibody (ab66544)
    Western blot - Anti-TFPI antibody (ab66544)
    All lanes : Anti-TFPI antibody (ab66544) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

References for Anti-TFPI antibody (ab66544)

This product has been referenced in:
  • Liczko J  et al. Tissue factor and tissue factor pathway inhibitor in chronically inflamed gallbladder mucosa. Biomed Res Int 2014:403639 (2014). IHC-Fr ; Human . Read more (PubMed: 24716194) »

See 1 Publication for this product

Publishing research using ab66544? Please let us know so that we can cite the reference in this datasheet.

Product Wall

Submit an abreview for ab66544 Submit a question for ab66544

3 Abreviews or Q&A

10
Votes: Highest First

Thank you again for your reply and suggestions. I'm currently
testing de-glycosylation of the samples and hopefully this will
show which of the bands or if both of them are correct.
The rTFPI control is from another company and ...

Read More

Thank you for your reply.
Thank you for trying some of the tips. Please do let me know what the results are once you have them. I'm curious myself, as well as want to ensure the antibodies are working as expected.

Should it turn out that...

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1) My samples are supernatants from Sum102 breast cancer cells
treated with PI-PLC (2 hours) to cleave off GPI-anchored TFPI.
These cells express a lot of TFPI and I would expect to see more
TFPIbeta in the + PIPLC samples than in the - ...

Read More

Thank you for your reply and the additional information.

I have a couple more questions as well as a few suggestions. I hope you don't mind.

1) Is the rTFPI that you were showing on the left hand side glycosylated or not?
2) ...

Read More

I have a question regarding your TFPI
antibodies that I have been using. When I use your C-terminal specific
antibody, AB9881 which should only detect TFPIalpha, I get strongest
bands
at around 45-46kDa. However, when I use the N-te...

Read More

Thank you for contacting us.

Antibody ab9881, detects only the alpha isoform.
Antibody ab66544, would detect both isoforms if they are present
For antibody ab48648, we do not know where the epitope is, thus we cannot predict if eit...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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