- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-TFPI antibody
See all TFPI primary antibodies -
DescriptionRabbit polyclonal to TFPI
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Frmore details
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Species reactivityReacts with: Human
Predicted to work with: Rat, Cow, Chimpanzee, Rhesus monkey, Orangutan
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TFPI.
(Peptide available as ab66543.)
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Positive control
- This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat, A431, HEK293, HepG2, MCF7, SHSY-5Y and U2OS.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab66544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 35 kDa). | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 24716194 |
Target
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FunctionInhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
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Tissue specificityMostly in endothelial cells.
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Sequence similaritiesContains 3 BPTI/Kunitz inhibitor domains.
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DomainThis inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
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Post-translational
modificationsO-glycosylated. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 7035 Human
- Entrez Gene: 29436 Rat
- Entrez Gene: 613026 Rhesus monkey
- Omim: 152310 Human
- SwissProt: P10646 Human
- SwissProt: Q02445 Rat
- SwissProt: Q28864 Rhesus monkey
- Unigene: 516578 Human
see all -
Alternative names
- Anti convertin antibody
- EPI antibody
- Extrinsic pathway inhibitor antibody
see all
Images
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Western blot - Anti-TFPI antibody (ab66544)All lanes : Anti-TFPI antibody (ab66544) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 52 kDa (why is the actual band size different from the predicted?)
This protein contains a number of potential glycosylation sites (SwissProt). The 52 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to TFPI.
References
This product has been referenced in:
- Liczko J et al. Tissue factor and tissue factor pathway inhibitor in chronically inflamed gallbladder mucosa. Biomed Res Int 2014:403639 (2014). IHC-Fr ; Human . Read more (PubMed: 24716194) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"