Recombinant Anti-TFPI2 antibody [EPR14442] (ab186747)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14442] to TFPI2
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TFPI2 antibody [EPR14442]
See all TFPI2 primary antibodies -
Description
Rabbit monoclonal [EPR14442] to TFPI2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human kidney tissue sections. WB: Jurkat, MCF7, rat spleen, HeLa, mouse heart, mouse brain, mouse kidney and mouse spleen lysates. IP: TFPI2 IP in HeLa cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14442 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- Jurkat whole cell lysate (ab7899)
- Mouse spleen tissue lysate - total protein (ab7937)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/40 - 1/60.
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WB |
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IP
1/40 - 1/60. |
WB
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin. -
Tissue specificity
Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy. -
Sequence similarities
Contains 3 BPTI/Kunitz inhibitor domains. -
Domain
This inhibitor contains three inhibitory domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7980 Human
- Entrez Gene: 21789 Mouse
- Entrez Gene: 286926 Rat
- Omim: 600033 Human
- SwissProt: P48307 Human
- SwissProt: O35536 Mouse
- Unigene: 438231 Human
- Unigene: 25612 Mouse
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Alternative names
- Placental protein 5 antibody
- PP5 antibody
- REF1 antibody
see all
Images
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All lanes : Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/5000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/10000 dilution
Lane 1 : Rat spleen lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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Immunohistochemical analysis of paraffin embedded human kidney tissue sections labeling TFPI2 using ab186747 at a 1/250 dilution. A prediluted HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Lysate from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells was immunoprecipitated with ab186747 at a 1/50 dilution. For the subsequent blot, ab186747 was used at a 1/1000 dilution, with a 1/1000 dilution of Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated as the secondary antibody. Blocking/ Dilution buffer: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (6)
ab186747 has been referenced in 6 publications.
- Wang ZY et al. PARP1 deficiency protects against hyperglycemia-induced neointimal hyperplasia by upregulating TFPI2 activity in diabetic mice. Redox Biol 46:102084 (2021). PubMed: 34364219
- Mo J et al. TFPI2 Promotes Perivascular Migration in an Angiotropism Model of Melanoma. Front Oncol 11:662434 (2021). PubMed: 34249699
- Fu H et al. Identification of the Recombinant Plasmodium vivax Surface-Related Antigen as a Possible Immune Evasion Factor Against Human Splenic Fibroblasts by Targeting ITGB1. Front Cell Dev Biol 9:764109 (2021). PubMed: 34938733
- Andresen MS et al. Indirect regulation of TFPI-2 expression by miR-494 in breast cancer cells. Sci Rep 10:4036 (2020). PubMed: 32132611
- Karmakar D et al. E2F5 promotes prostate cancer cell migration and invasion through regulation of TFPI2, MMP-2 and MMP-9. Carcinogenesis 41:1767-1780 (2020). PubMed: 32386317
- Tsuchiya H & Oura S Involvement of MAFB and MAFF in Retinoid-Mediated Suppression of Hepatocellular Carcinoma Invasion. Int J Mol Sci 19:N/A (2018). PubMed: 29757260