Overview

  • Product nameAnti-TGF alpha antibody
    See all TGF alpha primary antibodies
  • Description
    Rabbit polyclonal to TGF alpha
  • Tested applicationsSuitable for: WB, Neutralising, Sandwich ELISA, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Highly pure (>98%) recombinant hTGF alpha (human Transforming Growth Factor alpha).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hTGF alpha (0.5 ng/ml), a concentration of 2.5 - 2.7 µg/ml of this antibody is required.
Sandwich ELISA Use at an assay dependent concentration.

To detect Human TGF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. The antibody, in conjunction with biotinylated Anti-Human TGF-α ab83159 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TGF-α.

ELISA Use a concentration of 0.5 µg/ml.
IHC-P Use a concentration of 0.25 µg/ml.

Target

  • FunctionTGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
  • Tissue specificityIsoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.
  • Sequence similaritiesContains 1 EGF-like domain.
  • Cellular localizationCell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • EGF like TGF antibody
    • EGF-like TGF antibody
    • ETGF antibody
    • TFGA antibody
    • TGF A antibody
    • TGF type 1 antibody
    • TGF-alpha antibody
    • Tgfa antibody
    • TGFA_HUMAN antibody
    • Transforming growth factor alpha antibody
    • Transforming growth factor alpha precursor antibody
    • Wa1 antibody
    • Waved 1 antibody
    see all

Anti-TGF alpha antibody images

  • ab9585 at 0.25 µg/ml staining human breast invasive ductal carcinoma tissue by IHC-P.  The antibody was incubated for two hours at room temperature.  An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer.

References for Anti-TGF alpha antibody (ab9585)

This product has been referenced in:
  • Zhang HB  et al. miR-143 suppresses the proliferation of NSCLC cells by inhibiting the epidermal growth factor receptor. Exp Ther Med 12:1795-1802 (2016). WB . Read more (PubMed: 27602093) »
  • Dreymueller D  et al. Smooth muscle cells relay acute pulmonary inflammation via distinct ADAM17/ErbB axes. J Immunol 192:722-31 (2014). Mouse . Read more (PubMed: 24342803) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"