Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)

Overview

  • Product nameAnti-TGF beta 1 + 2 antibody [EPR5443]
  • Description
    Rabbit monoclonal [EPR5443] to TGF beta 1 + 2
  • Specificityab124894 recognises TGF beta 1 and 2.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human TGF beta 1. (Uniprot: P01137)

  • Positive control
    • A549 lysate, K562 lysate and L929 lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44, 48 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • RelevanceFunction: TGF beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. The inactive form consists of a TGFB1 homodimer non-covalently linked to a latency-associated peptide (LAP) homodimer. The inactive complex can contain a latent TGFB1-binding protein. The active form is a homodimer of mature TGFB1; disulfide-linked. Heterodimers of TGFB1/TGFB2 have been found in bone. Interacts with CD109 and DPT. Interacts with ASPN. Function: TGF beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Tissue specificity: TGF beta 1 is highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. Similarity: Both belong to the TGF-beta family.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • TGF-beta-1 antibody
      • TGF-beta-2 antibody
      • Transforming growth factor Beta 1 antibody
      • Transforming growth factor Beta 2 antibody

    Anti-TGF beta 1 + 2 antibody [EPR5443] images

    • All lanes : Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894) at 1/1000 dilution

      Lane 1 : A549 lysate
      Lane 2 : K562 lysate
      Lane 3 : L929 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 44, 48 kDa
    • All lanes : Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894) at 1/1000 dilution

      Lane 1 : TGF beta 2 recombinant protein
      Lane 2 : TGF beta 1 recombinant protein

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 44, 48 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)

    ab124894 has not yet been referenced specifically in any publications.

    Product Wall


    The lab let me know the following regarding their testing in mouse and rat:
    Even though there is 100% sequence homology with the mouse and rat proteins, we still typically test for cross reactivity before recommending them for use with other s...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"