Overview

  • Product nameAnti-TGF beta 1 antibody [2Ar2]
    See all TGF beta 1 primary antibodies
  • Description
    Mouse monoclonal [2Ar2] to TGF beta 1
  • Tested applicationsSuitable for: IHC-FoFr, WB, ELISA, IHC-Fr, ICC, Neutralising, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant human TGF beta 1 and latent TGF beta 1 expressed from CHO cells.

  • Positive control
    • HT2 cells, RAW 264.7 cells, PC-3 cells, Mouse and rat kidney tissue, mouse and rat liver tissue, Human liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab64715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/250.
WB Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

The detection limit for hTGF beta 1 is ~5ng/lane under non-reducing conditions. Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.

ELISA Use a concentration of 2 - 8 µg/ml. Using 100µl/well will detect ~30-2000pg/ml
IHC-Fr Use a concentration of 8 - 25 µg/ml.
ICC Use a concentration of 8 - 25 µg/ml.
Neutralising Use a concentration of 0.3 - 1 µg/ml. This dilution will neutralize 50% of the bioactivity due to 0.25ng/ml of rhTGF beta 1, using the HT2 cell line.
Flow Cyt Use a concentration of 25 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Post-translational
    modifications
    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cartilage-inducing factor antibody
    • CED antibody
    • Differentiation inhibiting factor antibody
    • DPD1 antibody
    • LAP antibody
    • Latency-associated peptide antibody
    • Prepro transforming growth factor beta 1 antibody
    • TGF beta 1 antibody
    • TGF beta antibody
    • TGF beta 1 protein antibody
    • TGF-beta 1 protein antibody
    • TGF-beta-1 antibody
    • TGF-beta-5 antibody
    • TGF-beta1 antibody
    • TGFB antibody
    • Tgfb-1 antibody
    • tgfb1 antibody
    • TGFB1_HUMAN antibody
    • TGFbeta antibody
    • TGFbeta1 antibody
    • Transforming Growth Factor b1 antibody
    • Transforming Growth Factor beta 1 antibody
    • Transforming growth factor beta 1a antibody
    • transforming growth factor beta-1 antibody
    • transforming growth factor, beta 1 antibody
    • Transforming Growth Factor-ß1 antibody
    see all

Anti-TGF beta 1 antibody [2Ar2] images

  • All lanes : Anti-TGF beta 1 antibody [2Ar2] (ab64715) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Mouse liver tissue lysate
    Lane 6 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.


    Performed under non-reducing conditions.

    Predicted band size : 45 kDa
    Additional bands at : 52 kDa (possible glycosylated form).

    Tissue lysates were denatured for 10-15 minutes at 90ºC. Blot was performed under non-reducing conditions. ab64715 was incubated overnight at 4ºC and the secondary antibody for 1 hour at RT.

  • ab64715 inhibits the mIL-4-dependent 3H-thymidine incorporation by HT-2 cells in a dose-dependent manner. The ED50 for this effect is typically 0.02-0.06ng/ml.
  • Approximately 0.3-1ug/ml of ab64715 will neutralize 50% of the bioactivity due to 0.25ng/ml of rhTGF-b1.
  • Human PC-3 cells were stained with ab64715 (filled histogram) or isotype control antibody (open histogram) followed by IgG (APC) anti-mouse antibody.
  • IHC-FoFr image of TGF beta 1 staining on sections from rat brain using ab64715 (1:250). The rat was treated with LPS and focal brain injury. 72 hours after injury, the rat was perfused with 4%PFA in 0.1M PBS. Antibody staining was performed on sections using free floating technique.

    See Abreview

  • ab64715 staining TGF beta 1 in mouse liver tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/50 in 1% BSA) at 4°C for 12 hours. An undiluted HRP-conjugated polyclonal to mouse IgG was used as secondary antibody.

    See Abreview

References for Anti-TGF beta 1 antibody [2Ar2] (ab64715)

This product has been referenced in:
  • Ueno K  et al. Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts. Sci Rep 6:28538 (2016). IHC-P ; Mouse . Read more (PubMed: 27329845) »
  • Zhang ZH  et al. An integrated lipidomics and metabolomics reveal nephroprotective effect and biochemical mechanism of Rheum officinale in chronic renal failure. Sci Rep 6:22151 (2016). WB ; Rat . Read more (PubMed: 26903149) »

See all 21 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (skin)
Permeabilization No
Specification skin
Blocking step goat serum followed by affinity purified F(ab) fragment anti-mouse IgG and Fc blocker as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Acetone
Username

Abcam user community

Verified customer

Submitted Mar 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.1 M PBS with 1% Triton X
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 12 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Denaturing (10% SDS-polyacrylamide gel)
Sample Dog Cell lysate - whole cell (adipose tissue derived mesenchymal stem cell)
Specification adipose tissue derived mesenchymal stem cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. SeungHoon Lee

Verified customer

Submitted Apr 09 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Non-reduced Denaturing
Sample Mouse Cell lysate - whole cell (HT2)
Specification HT2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 5°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0 (Abcam protocol)
Sample Pig Tissue sections (Spleen)
Specification Spleen
Permeabilization No
Fixative 10% NBF
Username

Abcam user community

Verified customer

Submitted Oct 11 2013

Thank you for contacting Abcam.

We spoke earlier regarding the expected western blot band size associated with TGF beta-1, detected by antibody ab64715. As we discussed, the molecular weight of the TGF beta-1 protein is about 45 kDa, which is ...

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Hello!

SInce it would be difficult to determine 100% if it is the specific band for TGF beta 1, I would re-run the gel. It is listed under the WB application notes on the datasheet that non-reducing conditions should be used, and we do not hav...

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Thank you for contacting us.

Ab64715 recognizes human TGF-b1 both in its active form and in its latent form when it is complexed with LAP (latency associated peptide.).

Active TGF-beta1 will run at 25kD under non-reducing Western Bl...

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It is always a pleasure to help.

Have a great evening as well.

Thank you for providing that information.

While we suggest a number of cell lines as positive control,HT2 cells, RAW 264.7 cells, PC-3 cells, these would not be appropriate for IHC. I do see that we have published an image and information ...

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