Overview

  • Product nameAnti-TGF beta 1 antibody [2C5]
    See all TGF beta 1 primary antibodies
  • Description
    Mouse monoclonal [2C5] to TGF beta 1
  • SpecificityWestern blotting demonstrated that this antibody reacts with the dimeric (25 kDa) and monomeric (12.5 kDa.) forms of TGF beta under both non-reducing and reducing conditions respectively. TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. This antibody recognizes both human platelet-derived and recombinant TGF beta in ELISA.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    TGF beta 1 from human platelets.

Properties

Applications

Our Abpromise guarantee covers the use of ab18679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. This antibody reacts with the dimeric (25 kDa) and monomeric (12.5 kDa) forms of TGF beta. When used at an antibody concentration of 5-20 ng/mL, ab18679 will allow visualization of 100 ng/lane of TGF beta Detects a band of approximately 25 and 12.5 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
    • Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
    • Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
    • Sequence similaritiesBelongs to the TGF-beta family.
    • Post-translational
      modifications
      Glycosylated.
      The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CED antibody
      • DPD1 antibody
      • LAP antibody
      • Latency-associated peptide antibody
      • Prepro transforming growth factor beta 1 antibody
      • TGF beta 1 antibody
      • TGF beta antibody
      • TGF beta 1 protein antibody
      • TGF-beta 1 protein antibody
      • TGF-beta-1 antibody
      • TGF-beta-5 antibody
      • TGF-beta1 antibody
      • TGFB antibody
      • Tgfb-1 antibody
      • tgfb1 antibody
      • TGFB1_HUMAN antibody
      • TGFbeta antibody
      • TGFbeta1 antibody
      • Transforming Growth Factor b1 antibody
      • Transforming Growth Factor beta 1 antibody
      • Transforming growth factor beta 1a antibody
      • transforming growth factor beta-1 antibody
      • transforming growth factor, beta 1 antibody
      see all

    References for Anti-TGF beta 1 antibody [2C5] (ab18679)

    ab18679 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab18679.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"