Overview

  • Product nameAnti-TGF beta 1 antibody [2E6]
    See all TGF beta 1 primary antibodies
  • Description
    Mouse monoclonal [2E6] to TGF beta 1
  • SpecificityWestern blotting demonstrated that this antibody reacts with the dimeric (25 kDa) and monomeric (12.5 kDa.) forms of TGF-beta under both non-reducing and reducing conditions respectively. TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. This antibody recognizes both human platelet-derived and recombinant TGF-beta in ELISA.
  • Tested applicationsSuitable for: Neutralising, WB, ELISAmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human TGF beta 1. TGF beta 1 from human platelets.

  • Positive control
    • WB: recombinant protein

Properties

Applications

Our Abpromise guarantee covers the use of ab647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising Use at an assay dependent concentration. PubMed: 17574548
WB Use a concentration of 1 - 10 µg/ml.

Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.This antibody, used at this concentration will allow visualization of 100 ng/lane of TFG-beta.

ELISA Use at an assay dependent concentration.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
    • Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
    • Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
    • Sequence similaritiesBelongs to the TGF-beta family.
    • Post-translational
      modifications
      Glycosylated.
      The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CED antibody
      • DPD1 antibody
      • LAP antibody
      • Latency-associated peptide antibody
      • Prepro transforming growth factor beta 1 antibody
      • TGF beta 1 antibody
      • TGF beta antibody
      • TGF beta 1 protein antibody
      • TGF-beta 1 protein antibody
      • TGF-beta-1 antibody
      • TGF-beta-5 antibody
      • TGF-beta1 antibody
      • TGFB antibody
      • Tgfb-1 antibody
      • tgfb1 antibody
      • TGFB1_HUMAN antibody
      • TGFbeta antibody
      • TGFbeta1 antibody
      • Transforming Growth Factor b1 antibody
      • Transforming Growth Factor beta 1 antibody
      • Transforming growth factor beta 1a antibody
      • transforming growth factor beta-1 antibody
      • transforming growth factor, beta 1 antibody
      see all

    References for Anti-TGF beta 1 antibody [2E6] (ab647)

    This product has been referenced in:
    • Rocks N  et al. ADAMTS-1 metalloproteinase promotes tumor development through the induction of a stromal reaction in vivo. Cancer Res 68:9541-50 (2008). WB ; Human . Read more (PubMed: 19010931) »
    • Schmid SA  et al. Lactate adversely affects the in vitro formation of endothelial cell tubular structures through the action of TGF-beta1. Exp Cell Res 313:2531-49 (2007). Read more (PubMed: 17574548) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Guinea pig Tissue lysate - whole (Brain)
    Loading amount 30 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing (12% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Yvonne Sondy

    Verified customer

    Submitted May 21 2012

    Thank you for your reply.

    I am sorry about the misunderstanding about the testing discount codes and I will make an exception this time due to the misunderstanding, however in future we do ask that you contact us prior to purchasing an antibo...

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    Thank you for contacting Abcam.

    Normally we issue you the testing discount code before you purchase the antibody. From the order information you provided, we sent you ab647 as a free of charge replacement for ab9758. When my colleague sent yo...

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    Thank you for your enquiry.
    I can confirm that ab647 (anti-TGF beta 1 antibody) has been tested and characterized for detecting sheep and human TGF beta 1.
    ab50036 is a recombinant human, (mature) protein expressed in mammalian A293 cells. Th...

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    Please find attached the certificates of compliance for ab647 and ab27969. If there is anything else I can help you with, please let me know.

    Please find below the information I was able to find: ab647 TGF beta antibody [2E6] had been demonstrated to have neutralization activity in the original paper: Schmid SA et al. Lactate adversely affects the in vitro formation of endothelial cell t...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (Fibroblast)
    Loading amount 30 µg
    Specification Fibroblast
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Nov 21 2006

    Thank you very much for providing me with the detailed protocol, it is very evident that the antibody does not work in IHC-P and we have immediately amended our datasheet to reflect this. I would like to send you a free vial of a different antibody, ab...

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    Thank you for the details regarding your protocol. I have a few more questions which would really help me get to the bottom of your problem: -what wax removal steps do you use? -the H2O2 step: what is the buffer? -the methanol step: is it i...

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    Thank you for your patience. The cap on the antibody vial should not have been loose and I do apologize for that. I'm sending you a replacement vial free of charge; for your record it is order# 43618 and you will receive the antibody this week. Please ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"