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Recombinant human TGF beta 1 and latent TGF beta 1 expressed from CHO cells.
Our Abpromise guarantee covers the use of ab64715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
The detection limit for hTGF beta 1 is ~5ng/lane under non-reducing conditions. Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
|ELISA||Use a concentration of 2 - 8 µg/ml. Using 100µl/well will detect ~30-2000pg/ml|
|IHC-Fr||Use a concentration of 8 - 25 µg/ml.|
|Neutralising||Use a concentration of 0.3 - 1 µg/ml. This dilution will neutralize 50% of the bioactivity due to 0.25ng/ml of rhTGF beta 1, using the HT2 cell line.|
|Flow Cyt||Use a concentration of 25 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Tissue lysates were denatured for 10-15 minutes at 90ºC. Blot was performed under non-reducing conditions. ab64715 was incubated overnight at 4ºC and the secondary antibody for 1 hour at RT.