The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 2 µg/ml. Determined for 20 ng recombinant human TGF Beta 1 under non-reducing conditions. Predicted molecular weight: 47 kDa for the unprocessed precursor; 25 kDa for the mature protein.
ELISA: Use at a concentration of 2 - 4 µg/ml. Determined for 9 pg/ml of recombinant human TGF beta 1.
Inhib: Use at a concentration of 0.3 - 1 µg/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
Involvement in disease
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
Belongs to the TGF-beta family.
Glycosylated. The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
Secreted > extracellular space > extracellular matrix.
Soller EC et al. Common features of optimal collagen scaffolds that disrupt wound contraction and enhance regeneration both in peripheral nerves and in skin. Biomaterials33:4783-91 (2012).
Read more (PubMed: 22483241) »
Wang L et al. Concentration-dependent effects of transforming growth factor ß1 on corneal wound healing. Mol Vis17:2835-46 (2011).
Read more (PubMed: 22128231) »